{"id":339,"date":"2016-11-03T17:37:59","date_gmt":"2016-11-03T17:37:59","guid":{"rendered":"http:\/\/lawyer2.mythemecloud.io\/?page_id=339"},"modified":"2022-01-27T09:59:21","modified_gmt":"2022-01-27T09:59:21","slug":"services","status":"publish","type":"page","link":"https:\/\/pt-neuranet.cnc.uc.pt\/?page_id=339","title":{"rendered":"Services"},"content":{"rendered":"\t\t
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\"\"<\/figure>

Measuring neuronal, synaptic and network activity<\/h3>

in vitro \/ ex vivo<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Recordings from neurons (cultured\/brain slices), axons, peripheral nerves,\u00a0other amenable preparations; studies of neuronal and synaptic activity and\u00a0<\/span>neuromodulation; field recordings in brain slices to study synaptic\u00a0<\/span>activity\/plasticity; calcium signaling, microfluidics and Multi-Electrode\u00a0<\/span>Arrays (MEAs).<\/span><\/p>

CEDOC\/NMS; CNC; i3S; iBiMED-UA; ICBAS\/MedinUP; ICVS; iMM\/FMUL;\u00a0UC (FMUC\/MIA\/CFE)<\/span><\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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\"\"<\/figure>

Pharmacological testing\/modulation<\/h3>

in vitro \/ ex vivo \/ in vivo<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Experimental design and electrophysiological characterization of the\u00a0functional\/pharmacological effects of molecules acting on receptors, ion\u00a0<\/span>channels or other cellular targets, using heterologous expression systems,\u00a0<\/span>primary cultures, IPSCs, brain slices or by intracerebral administration.<\/span><\/p>

\u00a0<\/span><\/p>

CEDOC\/NMS; CNC; iBiMED-UA; ICBAS\/MedinUP; iMM\/FMUL; UC\u00a0(FMUC)<\/span><\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\"\"<\/figure>

Electrochemical recordings of neuronal activity<\/h3>

in vitro \/ ex vivo \/ in vivo<\/span><\/span><\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Real-time monitoring of neurotransmitters and neuromodulators\u00a0(ATP\/aden sine, biogenic amines, nitric oxide, hydrogen peroxide) in vivo <\/em><\/span>(anesthetized and awake behaving rodents) and in vitro\/ex vivo<\/em> (cultured\u00a0<\/span>cells\/brain slices) using micro(bio)sensors coupled to fast electrochemical <\/span>techniques.<\/span><\/p>

CEDOC\/NMS; CNC; i3S; ICBAS\/MedinUP; UC (MIA\/CFE)<\/span><\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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\"\"<\/figure>

Optogenetic and chemogenetic manipulation of neuronal and glial activity<\/h3>

in vitro \/ ex vivo \/ in vivo<\/span><\/span><\/span><\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Circuit mapping using optogenetic and chemogenetic assisted methods ex\u00a0<\/em>vivo<\/em> and in vivo<\/em>. Manipulation of neuroglial populations by optogenetics\u00a0<\/span>and chemogenetics. Subcellular channelrhodopsin-assisted circuit\u00a0<\/span>mapping methods (sCRACM). Neuronal projection tracing.<\/span><\/p>

CEDOC\/NMS; Champalimaud Foundation; CNC; i3S; iBiMED-UA; ICVS;\u00a0UC (FMUC)<\/span><\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\"\"<\/figure>

In vivo electrophysiology, electrical stimulation and neural manipulation<\/h3>

in vivo<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Acute\/chronic electrophysiological recordings\/neuronal manipulation in\u00a0central or peripheral nerve tissue, in anesthetized, head-fixed or\u00a0<\/span>awake\/behaving animals; development and fabrication of electrodes and\u00a0<\/span>devices; stereotaxic surgeries for virus injection or electrode implantation.<\/span><\/p>

CEDOC\/NMS; Champalimaud Foundation; CNC; i3S; iBiMED-UA; ICVS;\u00a0iMM\/FMUL; UC (FMUC)<\/span><\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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\"\"<\/figure>

Imaging neuronal activity in vivo<\/h3>

in vivo<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Fluorescence imaging (calcium, neurotransmitters, or labeled molecules)\u00a0using fiber photometry, mini scopes and 2-photon microscopy in head-fixed\u00a0<\/span>awake animals; Acquisition of anatomical, hemodynamic, functional, and\u00a0<\/span>molecular signals using micro-ultrasound imaging system.<\/span><\/p>

\u00a0<\/span>Champalimaud Foundation; ICVS<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\"\"<\/figure>

Computational modeling and in silico neuroscience<\/h3>

in silico<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Construction and analysis of biophysically detailed models of neuronal\u00a0dynamics; Computational modeling of neuro-glial networks. Cross-region\u00a0<\/span>correlation and network data analysis and modeling.\u00a0<\/span>Implementation of advanced signal\/image analysis workflows (data\u00a0<\/span>analytics).<\/span><\/p>

CNC; i3S; ICVS<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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\"automation\"<\/figure>

Development of automated control of behavioural mazes for cognitive\nassessment<\/h3>

in vivo<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Development of automated systems for user-independent execution of\u00a0behavioral tasks of cognitive assessment in animal models (integration of\u00a0<\/span>animal position, activity and behaviour, through image and\/or sensors, for\u00a0<\/span>automated delivery of sensory or optogenetic stimulation).<\/span><\/p>

i3S; ICVS<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\"\"<\/figure>

Training in brain surgeries<\/h3>

in vivo<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Training in stereotaxic microsurgery in rodents (and post-surgery care),\u00a0including implants of bio-sensors, microelectrodes, cannulas, optical fibers\u00a0<\/span>and others.<\/span><\/p>

CNC; ICVS; iMM\/FMUL; UC (FMUC)<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Training in electrochemistry<\/h3>

in vitro \/ in vivo<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Training in electrochemistry\/carbon fiber amperometry recordings, carbon\u00a0fiber and micro(bio)sensor construction and evaluation and data analysis.<\/span><\/p>

CNC; ICBAS\/MedinUP; UC (MIA)<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Training in electrophysiology and data analysis<\/h3>

in vitro \/ ex vivo \/ in vivo<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Training in whole-cell patch-clamp, field potentials and electrochemistry\u00a0recordings; in vivo\/ex vivo recordings of electrical activity and modulation of\u00a0<\/span>peripheral nerves; electrophysiology basics for medical students\/interns\u00a0<\/span>with focus on clinical application; data analysis.<\/span><\/p>

CEDOC\/NMS; CNC; i3S; iBiMED-UA; ICBAS\/MedinUP; ICVS; iMM\/FMUL;\u00a0UC (FMUC\/MIA\/CFE)<\/span><\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Training in cell\/circuit specific manipulation using optogenetics and chemogenetics<\/h3>

in vivo<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Training in circuit mapping using laser-scanning optogenetic\u00a0photostimulation in acute slices; training in subcellular\u00a0<\/span>channelrhodopsin-assisted circuit mapping methods (sCRACM), including\u00a0<\/span>experimental and analysis methods.<\/span><\/p>

Champalimaud Foundation; ICBAS\/MedinUP; ICVS<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Training in Computational Data Analysis<\/h3>

in silico<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Training in analysis methods and information extraction from\u00a0electrophysiology\/imaging data; concepts and methodologies in\u00a0<\/span>Computational Data Analysis applied to biological\/biomedical experimental\u00a0<\/span>data; Bonsai visual programming language together with theory and\u00a0<\/span>hands-on practise of neurophysiology.<\/span><\/p>

i3S; ICVS<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Measuring neuronal, synaptic and network activity in vitro \/ ex vivo Recordings from neurons (cultured\/brain slices), axons, peripheral nerves, other amenable preparations; studies of neuronal and synaptic activity and neuromodulation; field recordings in brain slices to study synaptic activity\/plasticity; calcium signaling, microfluidics and Multi-Electrode Arrays (MEAs). CEDOC\/NMS; CNC; i3S; iBiMED-UA; ICBAS\/MedinUP; ICVS; iMM\/FMUL; UC (FMUC\/MIA\/CFE) Pharmacological testing\/modulation in vitro \/ […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ocean_front_end_style_editor":"no","ocean_post_layout":"full-width","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"0","ocean_second_sidebar":"0","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"off","ocean_display_header":"default","ocean_header_style":"","ocean_center_header_left_menu":"0","ocean_custom_header_template":"0","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"0","ocean_menu_typo_font_family":"0","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"0","footnotes":""},"class_list":["post-339","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/pt-neuranet.cnc.uc.pt\/index.php?rest_route=\/wp\/v2\/pages\/339","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pt-neuranet.cnc.uc.pt\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pt-neuranet.cnc.uc.pt\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pt-neuranet.cnc.uc.pt\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pt-neuranet.cnc.uc.pt\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=339"}],"version-history":[{"count":41,"href":"https:\/\/pt-neuranet.cnc.uc.pt\/index.php?rest_route=\/wp\/v2\/pages\/339\/revisions"}],"predecessor-version":[{"id":1098,"href":"https:\/\/pt-neuranet.cnc.uc.pt\/index.php?rest_route=\/wp\/v2\/pages\/339\/revisions\/1098"}],"wp:attachment":[{"href":"https:\/\/pt-neuranet.cnc.uc.pt\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=339"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}